Explains the real-time PCR procedure, non-specific and specific detection and discusses real-time PCR versus traditional PCR.
PREMIER Biosoft
Very useful and complete manual that includes an overview of real-time PCR and its components, Real-time PCR analysis technology, fluorescence detection systems, Melting curve analysis, passive reference dyes, contamination prevention, multiplex real-time PCR, internal controls and reference genes and troubleshooting.
ThermoFisher
A website that describes and summarises all technical aspects involved in quantitative gene expression analysis using real-time qPCR & dPCR.
Gene Quantification