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GENERALITIES / PRIMERS

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Definition
PCR primers are short fragments of single stranded DNA (15-30 nucleotides in length) that are complementary to DNA sequences that flank the target region of interest. The purpose of PCR primers is to provide a “free” 3’-OH group to which the DNA polymerase can add dNTPs.
Source: http://www.eeescience.utoledo.edu/Faculty/Sigler/RESEARCH/Protocols
/PCR/PCR.pdf

 


L i n k s

· PCR Primer design and Reaction Optimisation
Molecular Biology Techniques Manual, Third Edition
Ed Rybicki
Department of Molecular and Cell Biology, University of Cape Town
 

· Design of Primers for Automated Sequencing
Describes the steps involved in Design of Primers for automated DNA sequencing and the major pitfalls to avoid.
DNA Squencing Core
/ University of Michigan
 

· Bioinformatic tools and guideline for PCR primer design
This review summarizes the general guidelines for primer design online.
File Format: PDF/ Adobe Acrobat
Kamel A. Abd-Elsalam (2003), African Journal of Biotechnology

· A critical review of PCR primer design algorithms and crosshybridization case study
This paper analyzes the algorithms and parameter weightings of commonly used primer design programs, such as PRIDE, PRIME+, DOPRIMER, PRIMO, Primer Master, and MEDUSA.
File Format: PDF/ Adobe Acrobat
F. John Burpo (2001), Biochemistry 218
 

· Primer design
Describes some points to remember in the design of primers and degenerate oligonucleotides
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The Board of Regents of the University of Wisconsin System
 


 


Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

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