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PCR primers are short fragments of single stranded DNA (15-30 nucleotides in length) that are complementary to DNA sequences that flank the target region of interest. The purpose of PCR primers is to provide a “free” 3’-OH group to which the DNA polymerase can add dNTPs.
Source: http://www.eeescience.utoledo.edu/Faculty/Sigler/RESEARCH/Protocols


L i n k s

· PCR Primer design and Reaction Optimisation
Molecular Biology Techniques Manual, Third Edition
Ed Rybicki
Department of Molecular and Cell Biology, University of Cape Town

· Design of Primers for Automated Sequencing
Describes the steps involved in Design of Primers for automated DNA sequencing and the major pitfalls to avoid.
DNA Squencing Core
/ University of Michigan

· Bioinformatic tools and guideline for PCR primer design
This review summarizes the general guidelines for primer design online.
File Format: PDF/ Adobe Acrobat
Kamel A. Abd-Elsalam (2003), African Journal of Biotechnology

· A critical review of PCR primer design algorithms and crosshybridization case study
This paper analyzes the algorithms and parameter weightings of commonly used primer design programs, such as PRIDE, PRIME+, DOPRIMER, PRIMO, Primer Master, and MEDUSA.
File Format: PDF/ Adobe Acrobat
F. John Burpo (2001), Biochemistry 218

· Primer design
Describes some points to remember in the design of primers and degenerate oligonucleotides
The Board of Regents of the University of Wisconsin System


Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

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