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Selected links about  Asymmetric PCR generalities, protocols and applications.

Asymmetric PCR is used to preferentially amplify one strand of the original DNA more than the other. It finds use in some types of sequencing and hybridization probing where having only one of the two complementary stands is ideal. PCR is carried out as usual, but with a great excess of the primers for the chosen strand. Due to the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required

Source:Polymerase chain reaction, Wikipedia, The Free Encyclopedia


G e n e r a l   L i n k s

Asymmetric PCR for ssDNA Production
Molecular Biology Techniques Manual,
University of Cape Town
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P r o t o c o l s

Mullins Molecular Retrovirology Lab,
University of Washington
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Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

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