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GENERALITIES / POLYMERASES

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Definition
- General term for enzymes that carry out the synthesis of nucleic acid, using a pre-existing nucleic acid template and appropriate nucleotides (viz. ribonucleotides for RNA and deoxyribonucleotides for DNA).
Source
:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf
 


L i n k s

· Thermostable DNA Polymerases
Describes the sources and properties of Taq, Pfu and Vent polymerases.
Biotechnology and Genetic Engineering
.

 

· Life at High Temperatures
Details the discovery and the characteristics of Thermus aquaticus and Taq polymerase.
Thomas D. Brock
University of Wisconsin-Madison
 

· Methods and reagents: Fidelity of DNA polymerases for PCR
Discusses the fidelity of polymerases used for amplification of DNA by the polymerase chain reaction.
Paul N. Hengen, National Cancer Institute
 

· PCR fidelity of Pfu DNA polymerase and other thermostable DNA polymerases
This paper compares the error rate of Pfu with an expanded number of PCR polymerases, including exo– Pfu, Deep Vent, Vent, UlTma and Taq.
File Format: PDF/ Adobe Acrobat
Janice Cline et al. (1996),
Nucleic Acids Res.
 

· Error Rates for Thermal Resistant DNA Polymerases
A list of
reported error rates from different Thermal DNA Polymerases (Taq,  KlenTaq,  Vent,  Deep Vent, Pfu and Replinase)
Originally compiled by Eric First and later posted to the bionet.molbio.methds-reagnts newsgroup by Paul Hengen

 

 


Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

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