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Glossary of terms Commonly used in the Polymerase Chain Reaction Technique

 PCR (Polymerase Chain Reaction)

- The polymerase chain reaction is a test tube system for DNA replication that allows a "target" DNA sequence to be selectively amplified, or enriched, several million-fold in just a few hours
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- PCR is a technique for amplifying a specific region of DNA, defined by a set of two "primers" at which DNA synthesis is initiated by a thermostable DNA polymerase. Usually, at least a million-fold increase of a specific section of a DNA molecule can be realized and the PCR product can be detected by gel electrophoresis. The regions amplified are usually between 150-3,000 base pairs in length.
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- A technique for making many copies of a specific DNA sequence. The reaction is initiated using a pair of short primer sequences which match the ends of the sequence to be copied. Thereafter, each cycle of the reaction copies the sequence between the primers. Primers can bind to the copies as well as the original sequence, so the total number of copies increases exponentially with time.
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Glossary of Biotechnology Terms

- A polymerase chain reaction, or PCR, allows a molecular biologist to create multiple copies of DNA without using a living organism for synthesis
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- A technique for copying the complementary strands of a target DNA molecule simultaneously for a series of cycles until the desired amount is obtained
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