TAIL-PCR ( Thermal asymmetric interlaced polymerase chain reaction
is a powerful tool for the recovery of DNA fragments adjacent to known sequences
Amplification of Insert End Sequences from Bacterial Artificial Chromosome
Clones by Thermal Asymmetric Interlaced PCR
Liu and Huang,1998
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A heat-stable DNA polymerase isolated from
the bacterium Therrnus aquaticus, used in PCR.
Taq polymerase ("Taq Pol," or simply "Taq")
is a thermostable polymerase used in polymerase chain reaction to check
for the presence or absence of a gene by amplifying a DNA fragment. First
isolated from Thermus aquaticus, a bacterium that lives in hot
springs and hydrothermal vents, Taq was identified as the first polymerase
able to withstand the denaturing conditions required during PCR. Its
enzymatic halflife (at 95 degrees Celsius) is 40 min. ...
Taq polymerase, Wikipedia, The Free Encyclopedia.
The specific piece of DNA or RNA to be
amplified by the PCR.
DNA target . Refers
to a particular DNA sequence that is the target of a particular action,
such as hybridization by a probe, amplification by PCR, cleavage by an
A molecule that serves as the
pattern for synthesising another molecule, e.g. a single-stranded DNA
molecule can be used as a template to synthesise the complementary
Touchdown PCR is another
modification of conventional PCR that may result in a reduction of nonspecific
amplification. It involves the use of an annealing temperature that is higher
than the target optimum in early PCR cycles. The annealing temperature is
decreased by 1°C every cycle or every second cycle until a specified or
'touchdown' annealing temperature is reached. The touchdown temperature is then
used for the remaining number of cycles. This allows for the enrichment of the
correct product over any non-specific product.
Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on
A mutation in which a large segment of one
chromosome breaks off and attaches to another chromosome.