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  PCR GLOSSARY: (T)

 


TAIL-PCR ( Thermal asymmetric interlaced polymerase chain reaction )

-TAIL-PCR is a powerful tool for the recovery of DNA fragments adjacent to known sequences
Definition from:
Efficient Amplification of Insert End Sequences from Bacterial Artificial Chromosome Clones by Thermal Asymmetric Interlaced PCR
Liu and Huang,1998

See more about
TAIL-PCR


Taq polymerase

- A heat-stable DNA polymerase isolated from the bacterium Therrnus aquaticus, used in PCR.
Definition from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-T/Taq_polymerase.html

- Taq polymerase ("Taq Pol," or simply "Taq") is a thermostable polymerase used in polymerase chain reaction to check for the presence or absence of a gene by amplifying a DNA fragment. First isolated from Thermus aquaticus, a bacterium that lives in hot springs and hydrothermal vents, Taq was identified as the first polymerase able to withstand the denaturing conditions required during PCR. Its enzymatic halflife (at 95 degrees Celsius) is 40 min. ...
Taq polymerase, Wikipedia, The Free Encyclopedia.


Target

- The specific piece of DNA or RNA to be amplified by the PCR.
Definition from:
http://www.lshtm.ac.uk/pmbu/staff/rmcnerney/homepage/glossary.html

- DNA target . Refers to a particular DNA sequence that is the target of a particular action, such as hybridization by a probe, amplification by PCR, cleavage by an enzyme, etc.

http://www.epochbio.com/technology/glossary.htm


Template

-
A molecule that serves as the pattern for synthesising another molecule, e.g. a single-stranded DNA molecule can be used as a template to synthesise the complementary nucleotide strand.
Definition from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf


Touchdown PCR

- Touchdown PCR is another modification of conventional PCR that may result in a reduction of nonspecific amplification. It involves the use of an annealing temperature that is higher than the target optimum in early PCR cycles. The annealing temperature is decreased by 1°C every cycle or every second cycle until a specified or 'touchdown' annealing temperature is reached. The touchdown temperature is then used for the remaining number of cycles. This allows for the enrichment of the correct product over any non-specific product.
Definition from:
Quality Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on Environmental Samples


Translocation

- A mutation in which a large segment of one chromosome breaks off and attaches to another chromosome.
Definition from:
Genome Glossary


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