A measurement of weight. There are one thousand nanograms in one
Nucleic Acid Sequence Based Amplification
( NASBA )
NASBA is an isothermal
nucleic acid amplification procedure based on target-specific primers and
probes, and the coordinated activity of THREE enzymes: AMV reverse
transcriptase, RNase H and T7 RNA polymerase. NASBA allows direct
detection of viral RNA by nucleic acid amplification.
PCR is a conventional PCR with a second round of amplification using a
different set of primers. This second set of primers is specific to a
sequence found within the DNA of the initial conventional PCR amplicon.
The use of a second amplification step with the "nested" primer set
results in a reduced background from products amplified during the initial
PCR due to the nested primers’ additional specificity to the region. The
amount of amplicon produced is increased as a result of the second round
of amplification and due to a reduction in any inhibitor concentrations.
Assurance/Quality Control Guidance for Laboratories Performing PCR
Analyses on Environmental Samples
very sensitive method for amplfication of DNA, which takes part of the
product of a single PCR reaction (after 30-35 cycles), and subjects it to
a new round of PCR using a different set of PCR primers which are nested
within the region flanked by the original primer pair
second PCR is performed on the product of an earlier PCR using primers
which are internal to the originals. This improves sensitivity without
second round amplification of an already PCR-amplified sequence using a
new pair of primers which are internal to the original primers. Typically
done when a single PCR reaction generates insufficient amounts of product.
or RNA. Each of these compounds consists of a backbone of sugar molecules
ribose for RNA and deoxyribose for DNA linked by single phosphate groups.
Attached to the sugars of the backbone are any of four nitrogenous bases,
A, T, C or G for DNA and A, U, C or G for RNA.
Mouse Genome Informatics
acids are biochemical macromolecules that are composed of nucleotide
chains, the genetic building blocks of life. DNA (deoxyribonucleic acid)
and RNA (ribonucleic acid) are the most well known types of nucleic acid,
and are found in living cells and viruses.
large molecule composed of nucleotide subunits.
monomer units that are used to make up the DNA molecule. Each has one of
the four bases, A, G, T, or C attached to a sugar phosphate that forms the
backbone of the DNA polymer.
subunit of DNA or RNA consisting of a nitrogenous base (adenine, guanine,
thymine, or cytosine in DNA; adenine, guanine, uracil, or cytosine in
RNA), a phosphate molecule, and a sugar molecule (deoxyribose in DNA and
ribose in RNA). Thousands of nucleotides are linked to form a DNA or RNA
- Nucleotides are the single units
that make up nucleic acids like RNA and DNA literally the building blocks