In gel electrophoresis, a lane is the
section of a gel below one well. The gels we are using have six wells, so
they have six corresponding lanes. When you look at a stained gel, all the
bands in one lane came from the same sample of DNA loaded into one well.
In molecular biology, a "library" is a
complex mixture of recombinant DNA molecules in a suitable cloning vector
representing either the entire genome of an organism (a genomic library)
or the messenger RNA population of a whole organism, cell type, or tissue
type (a cDNA library).
Mouse Genome Informatics
An unordered collection of clones
(i.e., cloned DNA from a particular organism) whose relationship to each
other can be established by physical mapping.
Ligase chain reaction (LCR)
A method of DNA amplification similar
to PCR. LCR differs from PCR because it amplifies the probe molecule
rather than producing amplicon through polymerization of nucleotides. Two
probes are used per each DNA strand and are ligated together to form a
single probe. LCR uses both a DNA polymerase enzyme and a DNA ligase
enzyme to drive the reaction. Like PCR, LCR requires a thermal cycler to
drive the rxn and each cycle results in a doubling of the target nucleic
acid molecule. LCR can have greater specificity than PCR.
Plural of locus.
The position of a gene,
DNA marker or genetic marker on a chromosome
A specific physical position on a
chromosome at which a gene or gene pair resides...
PCR in which the product is several kilobases
long. Using cocktails of enzymes products of up to 40 kb have been
Long PCR (i.e. amplification of DNA
fragments of several thousand base pairs) is now widely used in numerous
fundamental or applied scientific fields and represents an invaluable
diagnostic tool in molecular medicine.
Escherichia coli exonuclease III enhances long PCR amplification of
damaged DNA templates
Fromenty et al., 2000