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  PCR GLOSSARY:  (I)

 


Insertion

-
A type of mutation in which one or more nucleotides is inserted into a DNA sequence. Small insertions within a gene can alter the reading frame, and thus the amino acid sequence of the encoded protein.
Definition from:
Mouse Genome Informatics

- Addition of a DNA element into a chromosome
Definition from:
Hypermedia Glossary Of Genetic Terms


In situ PCR
- Definition from:

- In situ PCR is a powerful technique in which gene fragments are amplified within tissue sections.
Definition from:
Diagnostic value of in situ polymerase chain reaction in leprosy


Intercalating dyes (SYBR Green and similar)

- Real Time Amplification System. Despite being the most economical option for detection and quantifying real time amplified products, intercalating dyes (i.e. SYBR Green), bind to any double-stranded DNA present in the reaction, including primer-dimers and other nonspecific reaction products. This requires to take specific precautions. Therefore most of the protocols for kits based upon this technology include a melting curve analysis, intended to identify the amplified product that has been detected.
Definition from:
http://www.biotools.net/eng/tecnica/t33.htm


Intron

- Part of a gene whose sequence is transcribed but not present in a mature mRNA after splicing.
Definition from:
Mouse Genome Informatics

- DNA sequence that interrupts the protein-coding sequence of a gene; an intron is transcribed into RNA but is cut out of the message before it is translated into protein
Definition from:
Genome Glossary

- The DNA base sequence interrupting the protein-coding sequences of a gene; intron sequences are transcribed into RNA but are cut out of the message before it is translated into protein. ...
Definition from:
Hypermedia Glossary Of Genetic Terms


Inversion

- A type of mutation in which a length of DNA is broken in two positions and repaired in such a way that the medial segment is now present in reverse order. Inversions range in size from those large enough to be visible cytogenetically to those involving only a few base pairs.
Definition from:
Mouse Genome Informatics

- The term introduced by Sturtevant (1926) for the reversal of the linear sequence of the genes in a segment of a chromosome. In an inversion heterozygote one of the chromosomes contributed by one parent has an inverted segment while the homologous chromosome contributed by the other parent has the normal gene sequence
Definition from:
Hypermedia Glossary Of Genetic Terms


IPCR ( Inverse PCR )

-
Variation of PCR that makes the amplification of DNA segments of unknown sequence that flank DNA segments of known sequence possible; in brief, total DNA is digested to completion and fragments ligated under conditions that favour circularization of fragments; pair of PCR primers, designed from known sequence, are used to prime PCR from opposite strands resulting in amplification of fragment of unknown sequence.
Definition from:
Glossary of Biochemistry and Molecular Biology

- Inverse PCR is a method used to allow PCR when only one internal sequence is known. This is especially useful in identifying flanking sequences to various genomic inserts. This involves a series of digestions and self ligation before cutting by an endonuclease, resulting in known sequences at either end of the unknown sequence.
Definition from:
Polymerase Chain Reaction, Answers.com


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