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 PCR GLOSSARY:  (H)

 


Haplotype

- A set of closely linked genetic markers present on one chromosome which tend to be inherited together (not easily separable by recombination). Some haplotypes may be in linkage disequilibrium.
Definition from:
Hypermedia Glossary Of Genetic Terms

- A set of closely linked alleles (genes or DNA polymorphisms) inherited as a unit. A contraction of the phrase "haploid genotype". Different combinations of polymorphisms are known as haplotypes. Collectively the results from several loci could be referred to as a haplotype. "Haplo" comes from the Greek word for "single
Definition from:
Genetics/DNA Glossary


Heteroduplex

- DNA A double-stranded DNA molecule or a DNA/RNA hybrid where each strand is from a different source
Definition from:
Hypermedia Glossary Of Genetic Terms

- A DNA double helix formed by annealing single strands from different sources; if there is a sequence difference between the strands, the heteroduplex may show single strand loops or bubbles (unpaired regions).
Definition from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-H/heteroduplex.html


Heteroduplex analysis

-
The study of the mobility of heteroduplex DNA under polyacrylamide gel electrophoresis. The reduced mobility of heteroduplex DNA compared with homoduplex DNA is proportional to the degree of divergence of the sequences.
Definition from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf


Homoduplex

- DNA A double-stranded DNA molecule where both strands are from the same source.
Definition from:
Hypermedia Glossary Of Genetic Terms


Hot-start PCR

Is a modification of conventional PCR that reduces non-specific product amplification. In this procedure amplification cannot occur until the reaction temperature is above that where non-specific annealing of primers to targets occurs. This block in amplification is usually accomplished by using a DNA polymerase that is inactive until higher temperatures are reached.
Quality Assurance/Quality Control Guidance for Laboratories Performing PCR Analyses on Environmental Samples


Hybridization

- The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule. One strand is often labeled and used as a probe to detect the presence of the second strand.
Definition from:
Hypermedia Glossary Of Genetic Terms

- The joining together of complementary strands of DNA by the process of base pairing when the two strands are from different sources. For example, a DNA strand immobilized on a blot and its complementary radioactively labeled DNA probe.
Definition from:
genelex.com

- In molecular biology: the binding of sequences to form a double-stranded structure.
Definition from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf


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