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 PCR GLOSSARY: (C)

 


CAPS  (Cleaved Amplified Polymorphic Sequence) See PCR-RFLP
 


cDNA  (complementary DNA)

- DNA transcribed from an RNA molecule by an enzyme called reverse transcriptase.
Definition from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf


- A DNA sequence which was produced from mRNA by reverse transcription. A cDNA is so-called because its sequence is the complement of the original mRNA sequence. However, when double-stranded cDNA is synthesized, it contains both the original sequence and its complement.
http://www.cs.wustl.edu/~jbuhler/research/array/glossary.html
 


cDNA library 

-
A collection of cDNA's, each of which has been inserted in a DNA vector (e.g. a circular DNA plasmid) and replicated in a bacterium such as E. coli. The bacteria maintain a ready pool of the cDNA's and can be cultured to make copies of the library for many experiments. A population of bacteria containing a single inserted cDNA is called a clone. To extract a cDNA from the library, the insert from the corresponding clone can be amplified using PCR primed off the vector sequences connected to the ends of the cDNA
Definition from:
http://www.cs.wustl.edu/~jbuhler/research/array/glossary.html

- A pool of complementary DNA clones produced by cDNA cloning of total messenger RNA from a single source (cell type, tissue, embryo).
Definition from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-C/cDNA_library.html


Cloning

- Using specialized DNA technology to produce multiple, exact copies of a single gene or other segment of DNA to obtain enough material for further study. ...
Definition from:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-C/cloning.html

- In molecular biology: the process of using DNA manipulation procedures to produce multiple copies of a single gene or DNA segment
Definition from:
http://www.igd.cornell.edu/MolecularMarkers/Glossary.pdf

 


Cloning vector

- DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self- replication; vectors introduce foreign DNA into host cells, where it can be reproduced in large quantities. Examples are plasmids, cosmids, and yeast artificial chromosomes; vectors are often recombinant molecules containing DNA sequences from several sources.
Definition from:
http://linkage.rockefeller.edu/wli/glossary/genetics.html


Colony PCR

- Colony PCR is can be used after a transformation to screen colonies for the desired plasmid. Primers are used which generate a PCR product of known size. Thus, any colonies which give rise to an amplification product of the expected size are likely to contain the correct DNA sequence
Definition from:
OpenWetWare contributors, "Colony PCR," OpenWetWare, , http://openwetware.org/index.php?title=Colony_PCR&oldid=11695 (accessed August 26, 2006).

- Use of a bacterial colony for direct polymerase chain reaction to amplify a specific nucleotide sequence.
Definition from:
http://www.sci.sdsu.edu/~smaloy/Glossary/C.html


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