In gel electrophoresis, a band represents the dyed or stained molecule
you're interested in. In a sample containing two different sized pieces of
DNA, there will be two bands on the gel in that lane. The smaller piece of
DNA will move more quickly through the gel, so it will be farther from the
well. Each band is composed of hundreds of copies of the DNA molecule of
The chemical unit that
characterises a nucleotide. In DNA, the bases found are adenine (A),
guanine (G), thymine (T) and cytosine (C). In RNA, the bases are adenine,
guanine, uracil (U) and cytosine.
The molecular building blocks of DNA:
adenine (A), cytosine (C), guanine (G), thymine (T). In DNA, A attaches
only to T, and C attaches only to G.
pair ( bp )
Two nitrogenous bases
paired together in double-stranded DNA by weak bonds; specific pairing of
these bases (adenine with thymine and guanine with cytosine) facilitates
accurate DNA replication; when quantified (e.g., 8 bp), refers to the
physical length of a sequence of nucleotides
Buffer (PCR Buffer)
PCR buffer is necessary to
create optimal conditions for activity of Taq DNA polymerase. Buffers
often contain Tris-Hcl, KCl, and sometimes MgCl2. PCR buffers are often
available in 10X concentration and are sometimes Taq formulation-specific.