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  PCR GLOSSARY:(A)

 


AFLP
: (Amplified Fragment Length Polymorphism)

- Amplified fragment length polymorphism (AFLP) is a PCR-based technique that involves restriction of genomic DNA followed by ligation of adaptors to the fragments generated and selective PCR amplification of a subset of these fragments. The amplified fragments are separated on a sequencing gel and visualized by autoradiography or fluorescent sequencing equipment.
Definition from
:
A format for databasing and comparison of AFLP fingerprint profiles
Yan Hong and Aaron Chuah
, 2003
 

- AFLP-PCR was originally described by Zabeau & Vos in 1993. The procedure of this technique is divided into three steps: 1)Digestion of total cellular DNA with one or more restriction enzymes and ligation of restriction half-site specific adaptors to all restriction fragments. 2)Selective amplification of some of these fragments with two PCR primers that have corresponding adaptor and restriction site specific sequences. 3) Electrophoretic separation and amplicons on a gel matrix, followed by visualisation of the band pattern.
Definition from
:
http://insilico.ehu.es/AFLP/info.html


Alu-PCR

-
PCR using a primer that anneals to Alu repeats to amplify DNA located between two oppositely oriented Alu sequences. Used as a method of obtaining a fingerprint of bands from an uncharacterized human DNA
Definition from:
Human Molecular Genetics 2, Tom Strachan & Andrew P. Read


Amplicon

-
An amplicon is a small piece of DNA that has been amplified by PCR
Definition from:
http://www.bioexchange.com/news/news_page.cfm?id=8306

- The product of PCR or LCR; a piece of DNA that has been synthesized using amplification techniques
Definition from
:
http://www.biochem.northwestern.edu/holmgren/Glossary/Definitions/Def-A/amplicon.html
 


Amplification See DNA amplification
 


Annealing

-
Annealing is a process where the forward and reverse primers anneal to separated DNA template strand.
Definition from:http://www.ppsk.usm.my/lecturers/mravi/PDF_FIles/Group_3_2002.pdf

- Annealing is a bimolecular process, but there are competing processes in which both intrastrand and interstrand base pairs can form, ultimately leading to the stable duplex
Definition from:
http://www.biochem.arizona.edu/classes/bioc462/462a/NOTES/
Nucleic_Acids/nucacid_structure.html


Asymmetric PCR

-  Asymmetric PCR is used to preferentially amplify one strand of the original DNA more than the other. It finds use in some types of sequencing and hybridization probing where having only one of the two complementary stands is ideal. PCR is carried out as usual, but with a great excess of the primers for the chosen strand. Due to the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required
Definition from:
Polymerase chain reaction, Wikipedia, The Free Encyclopedia


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